Friday, August 5, 2016

Week 7 and 8: This is the End, Beautiful Friend

These weeks have been a grind of arranging for patient urine samples, processing them into single cell suspensions, and then assessing cell concentration and viability in the hopes of meeting the quality control conditions for Drop-seq processing. The high sample to sample variability has made this quite challenging, but I was finally able to submit a sample. It remains to be seen if the cDNA library generated meets QC for sequencing.

I was also able to work with a graduate student who set up the Drop-seq system for the genomics facility and learn some of the subtleties in the protocol. This was incredibly beneficial for me as I finish setting up my own Drop-seq in my lab.

Unfortunately, I ended up not observing an actual transplantation surgery, but this is probably for the best as the last time I was in an operating room I ended up passing out. My other experiences working in the research lab, shadowing in the clinic, sitting in on consults and grand rounds, and observing inpatient rounds have made this clinical immersion more than worthwhile.


Until next time, New York.

8 comments:

  1. Dear FRIENDS,
    now i have a beautiful smiling face and so are which you all, after reading my testimony. My name is jessica, I am 31 years old,
    And live with my boyfriend in Belgium, but I am UK. I have been diagnosed with pineal cyst since 2012, which the Doctors told me the only solution to this condition was surgery. I know how you feel and what you are going through, I don't have a baby though. I just want to tell you that I am cured now,with the help of doctor Williams herbal medication, the last time I can remember having pineal cyst was October this year. It was a long and difficult process but I am so thankful to dr williams I don't have it anymore. Please know you can be cured too. Accept what happened and is happening to you, forgive, let the past go, love yourself and others unconditionally and be thankful. Everything happens for a reason. If you want to be in contact with dr williams and share your own testimony and thoughts feel free to e-mail him on; drwilliams098675@gmail.com for advice and for his product.

    ReplyDelete
  2. I recently have some difficult conversations and I did what a lot of people do when they want to know more about something. I googled it. I came across Dr Williams herbal medicine on YouTube so many people thanking him about his good work. I also have pineal cyst. I wasn’t running all over God’s creation with every man I could find but here I am. I have felt bad about myself for so many years now because of my pineal cyst status. I obviously still have some self-accepting to do but I want to thank Dr Williams for everything he have done for my life ,after taking Dr Williams medicine i was completely free from pineal cyst within one month of usage, I think what you are doing is so admirable. you have helped me a lot! I want to definitely reach out to you and thank you for your amazing work. You are a good person, and an extremely talented man. You have helped millions with your herbs, and have really inspired me,and i pray you still continue doing the good work.you can also email him on drwilliams098675@gmail.com for help

    ReplyDelete
  3. DR WILLIAMS HERBAL CURE TO PINEAL CYST
    I can’t believe this. A great testimony that i must share to all pineal cyst patient in the world. i never believed that their could be any complete cure for pineal cyst with out going for surgery ,i saw people testimony on blog sites and Facebook page of how Dr Williams prepare herbal cure for different king of illness,and with the help of his herb they where able to cure there pineal cyst naturally. i had to try it too and you can,t believe that in just few weeks i started using it all my pains stop gradually . Right now i want to tell you all that am now completely free from pineal cyst,and on thin now i have not had any pain, and i have just went for text last week and the doctor confirmed that there is no trace of any pineal cyst. Glory be to God for leading me to this great Dr Williams I am so happy as i am sharing this testimony. My advice to you all who thinks that their is no herbal cure for pineal cyst that is Not true ,just contact him and get cure from his email drwilliams098675@gmail.com and you will be free and free forever, Try it and you will not regret it because it truly works. i hope to see your testimony soon.

    ReplyDelete
  4. Looking for natural medicine to pineal cyst without any side effect Dr. Williams is the best because it has no side effect, After my son being diagnosed of pineal cyst in 2010, I have used numerous medicine for him with no improvement. He spent most of the summer in the hospital. I remember him feeling abandoned and terrified of the many injection needles that seemed necessary to keep him calm. All Western Medicine used in the past did not have any noticeable difference,one day as i was going through the internet i came across so many people with different disease testifying about dr williams herbal remedies,i ordered for my son pineal cyst on line through dr williams,that's when he started using dr williams Herbal Medicine,and now is completely cured from pineal cyst , his medicine is really a permanent cure to pineal cyst, for more info contact (drwilliams098675@gmail.com for advice and for his product

    ReplyDelete
  5. Dear FRIENDS,
    now i have a beautiful smiling face and so are which you all, after reading my testimony. My name is jessica, I am 31 years old,
    And live with my boyfriend in Belgium, but I am UK. I have been diagnosed with pineal cyst since 2012, which the Doctors told me the only solution to this condition was surgery. I know how you feel and what you are going through, I don't have a baby though. I just want to tell you that I am cured now,with the help of doctor Williams herbal medication, the last time I can remember having pineal cyst was October this year. It was a long and difficult process but I am so thankful to dr williams I don't have it anymore. Please know you can be cured too. Accept what happened and is happening to you, forgive, let the past go, love yourself and others unconditionally and be thankful. Everything happens for a reason. If you want to be in contact with dr williams and share your own testimony and thoughts feel free to e-mail him on; drwilliams098675@gmail.com for advice and for his product.

    ReplyDelete
  6. ALL THANKS TO DR WILLIAMS WITH HIS HERB MY WIFE WAS COMPLETELY CURED FROM PINEAL CYST.
    Is my pleasure to comment on this site and i thank the admin of this site for his/her great work so far.one of the happiest moment in my life was when the doctor confirm that my wife is been free from this illness. this awesome moments makes me the happiest man on earth, I really don't know how to thank DR WILLIAMS for helping my wife get cured for over how many year of suffering from pineal cyst. i came across DR Williams contact through a headline news on internet about how DR Williams help a woman to get cured of her pineal cyst and so many other with similar body problem ,i contacted him and he told me how to get his herb,few day later he sent me the herbal portion which my wife take every morning for 28 days, and his medicine was able to cure her completely from pineal cyst. and now she very mush okay without any side effects whatsoever, If you have pineal cyst, you can contact him on his email address drwilliams098675@gmail.com for advice and for his product.

    ReplyDelete
  7. As a sign of gratitude for how my husband was saved from pineal cyst , i decided to reach out to those still suffering from this.
    My husband was diagnosed of pineal cyst, and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he had weakness of the body , and he always complain of ringing in ears (tinnitus) and headache . we tried various therapies prescribed by our neurologist but none could cure him.one faithful day i decided to go for a researched and i saw a testimony by someone who was cured and so many other with similar brain problem, and he left the contact of the doctor who had the cure to pineal cyst. I never imagine pineal cyst has a cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months time he was fully okay even up till this moment he is so full of life. pineal cyst has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my testimony

    ReplyDelete
  8. I am Sophie from Canada, I once suffered from a terrible and Chronic pineal cyst ,since i was 30 , the doctor told me there was no permanent cure i was given medications to slow down its progress, i constantly felt my health was deteriorating as i constantly go out of breath,and this illness was really terrible especially when am going out with my friends, i have this constant disorder for about 15 years, this was really a terrible ailment ,on thin one day that i was going through the internet,and i came across a post of Mrs Shelley on how his husband was been cured from pineal cyst through the help of Dr Williams herbal product, I contacted this herbal doctor via his email and explain everything to him and make purchase of his product,few days later he sent me the herbal medicine through courier service, when i received the herbal medicine, i used it for 1 months as prescribed by Dr Williams and i was totally cured within those week of usage,on thin now i have not experience any sign or characteristics again . for more information you can email him on drwilliams098675@gmail.com for help

    ReplyDelete